G4 Sequencing Platfom FAQs

The G4 directly outputs your sequencing data into the FASTQ format. The G4 uses a base caller that converts the fluorescent intensities directly into bases as your samples are sequenced. If you include the index information in your sample sheet for the run, the G4 can also automatically demultiplex your samples when the sequencing is complete. Alternatively, you can start (or restart) the demultiplex process with the Singular demultiplex software.  

The G4 output FASTQ files follow the standard 4-line FASTQ convention. Therefore, data generated on the G4 is compatible with most bioinformatic analytical pipelines and software that uses FASTQ files as input. You can learn more about the FASTQ format from Wikipedia.

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