Discover G4
A benchtop sequencing platform delivering accuracy, speed, power, and unprecedented flexibility for a wide range of genomic applications.
Accuracy
Reimagined Chemistry
At the core of the G4 is a proprietary 4-color Rapid SBS chemistry comprised of novel nucleotides, specifically-engineered enzymes, and molecular biology methods to deliver highly accurate paired-read sequencing.
Flexibility
4 Lanes per Flow Cell
Each flow cell is intentionally designed with 4 independent lanes, reducing sample pooling and offering flexibility to run multiple experiments in parallel.
Flexibility
Up to 4 Flow Cells per Run
The G4 delivers scalable run capacity to empower labs that have variable volume and time-sensitive samples with cost efficient, single-day turnaround times.
Speed
Daily Sequencing
Novel chemistry combined with breakthrough imaging technology and rapid fluidics reduces run times from days to hours.
Power
Unmatched Power in a Benchtop Sequencer
The G4 is engineered for throughput. With up to 2.5x more data output per hour than the leading benchtop instrument, the G4 will sequence up to 4 human genomes in a single day.
Workflow
G4 was designed to fit into any existing lab ecosystem. From compatibility with a wide range of sample preparation workflows through seamless integration into bioinformatic pipelines, G4 is a plug-and-play solution.
Prepare
- Compatibility with leading library preparation kits and workflows
- Prepare libraries with G4 adapters and indices, or convert existing libraries
- Electronic ID consumables for inventory management and traceability
Sequence
- Up to 4 flow cells with 4 individual lanes for maximum run flexibility
- Intuitive user interface for novice and advanced users
- Integrated clustering and automatic instrument wash
Analyze
- Real-time analysis metrics provided per lane and flow cell
- Demultiplexed FASTQ file output for seamless integration with bioinformatics pipelines
- Connectivity for cloud-based storage and remote customer service
G4 Specifications
| Reagent Configuration1 | Run Time2 | Paired Reads / Flow Cell3 | Paired Reads / Run3 | Quality4 | |
|---|---|---|---|---|---|
| F2 Flow Cell | 100 cycles | ~11 hours | 200M | 800M | >85% bases ≥ Q30 |
| 200 cycles | ~15 hours | ||||
| 300 cycles | ~19 hours | ||||
| F3 Flow Cell | 50 cycles | ~11 hours | 400M | 1.6B | |
| 100 cycles | ~14 hours | ||||
| 200 cycles | ~19 hours | ||||
| 300 cycles | ~24 hours | ||||
| Max Read5 | 28x91 Single Cell | ~30 hours | 800M | 3.2B | |
| 28x50 Spatial FFPE | ~24 hours | 1.2B | 4.8B |
- 1 Reagents include 50 additional cycles above what is represented to account for index sequencing needs.
- 2 Run time is measured from run start through clustering, sequencing, and instrument wash for non-indexed reads.
- 3 Paired reads passing filter for F2 and F3 are dependent on application and read length.
- 4 Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.
- 5 Currently only compatible with 10x Genomics Chromium™ 3′ and 5’ Gene Expression assays and Visium™ Spatial Gene Expression. Kits allow for 1 sample per lane.
G4 Applications
High sequencing throughput, modular run sizes, and lane-by-lane addressability enables users to process samples across a variety of applications.
| F2 Flow Cell Samples / Flow Cell | F3 Flow Cell Samples / Flow Cell | Max Read Samples / Flow Cell | Samples / Run | |
|---|---|---|---|---|
| RNA Gene Expression (2x50 bp, 10M paired reads) | 20 | 40 | — | 160 |
| RNA-Seq (2x100 bp, 50M paired reads) | 4 | 8 | — | 32 |
| Single Cell RNA-Seq (130 cycles, 7,500 cells/sample and 20,000 paired reads/cell) | 1 | 2 | 4 | 16 |
| Exome (2x150 bp, 34 Mb at 150x coverage) | 17 | 35 | — | 141 |
| Target Enrichment (2x150 bp, 800 Kb at 4,000x coverage) | 18 | 37 | — | 150 |
| Shotgun Metagenomics (2x150 bp, 10M paired reads) | 20 | 40 | — | 160 |
| Human Whole Genome (2x150 bp, 3 Gb at 30x coverage) | — | 1 | — | 4 |
- Sequencing multiplexing capabilities are approximations based on assumptions and configurations listed in table and expected, but not guaranteed, throughput on G4 flow cells. Results may vary based on experimental design and sample type. Please refer to kit specifications for more detail.
The World’s Most Powerful Benchtop Sequencer
The G4 is an innovative sequencing platform with novel high-performance chemistry and advanced engineering, optimized to deliver unmatched power and versatility.
Watch G4 Video
G4 Kits and Reagents
G4 kits are available in multiple configurations depending on read length and size requirements for maximum system flexibility and cost efficiency.
Introducing Max Read™ Kits
Delivering up to 3.2 billion reads per run, the Max Read Kit offers unparalleled cost and throughput for short read sequencing on a benchtop platform.
G4 Pricing & Availability
For additional details around G4 pricing and availability, please provide your information and a Singular Genomics representative will be in contact as soon as possible.
