Discover G4

A purposefully designed sequencing platform that delivers accuracy, speed, and unprecedented flexibility to power a wide range of genomic applications. 

G4 Machine

Accuracy

Novel Chemistry

At the core of the G4 is a proprietary 4-color SBS chemistry comprised of novel nucleotides, specifically-engineered enzymes, and molecular biology methods to deliver highly accurate paired-read sequencing.

flow cells zoomed in

Flexibility

Intentional Flow Cell Design

4 individual lanes per flow cell

With 4 independent lanes per flow cell, the G4 reduces sample pooling requirements and offers flexibility to run multiple experiments in parallel.

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Flow Cells in G4

Flexibility

Scalable Capacity

1-4 independent flow cells

The G4’s run modularity empowers labs that have variable volume and time-sensitive samples with cost efficient, single-day turnaround times.

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Speed

Breakthrough Imaging Technology

Novel chemistry combined with high-resolution imaging and rapid fluidics reduces run times from days to hours.

Power

Engineered for Throughput

With up to 3x more data output per hour than any bench top instrument, the G4 will sequence up to 4 human genomes in just 16-19 hours. 

G4 Machine

Workflow


The G4 was designed to fit into any existing lab ecosystem. From compatibility with a wide range of sample preparation workflows through seamless integration into bioinformatic pipelines, the G4 is a plug-and-play solution. 

1

Prepare

  • Compatibility with existing library prep workflows
  • Electronic ID consumables for inventory management and traceability 
  • Easy to load magnetic snap-in flow cells 
  • 4 individually addressable lanes per Flow Cell for Independent Experiments
2

Sequence

  • Intuitive user interface for novice and advanced users
  • On and off-instrument run creation
  • Automatic,  integrated clustering and instrument wash
  • Real-time remote run and system health monitoring
3

Analyze

  • High speed image analysis of patterned flow cells
  • High performance base calling powered by NVIDIA GPUs
  • Built-in demultiplexing
  • FASTQ file output for seamless integration with bioinformatic pipelines
  • Connectivity for cloud-based storage and remote customer service

G4 Specifications


F2 Kits F3 Kits*
Number of reads (clusters) 150-165 M 300-330 M
Sequencing Output (Base calls) 1 x 50 bp (50 cycles) 15-17 Gb
2 x 50 bp (100 cycles) 15-17 Gb 30-33 Gb
2 x 100 bp (200 cycles) 30-33 Gb 60-66 Gb
2 x 150 bp (300 cycles) 45-50 Gb 90-100 Gb
Run Time 1 x 50 bp (50 cycles) 6-8 hrs
2 x 50 bp (100 cycles) 8-10 hrs 8-10 hrs
2 x 100 bp (200 cycles) 12-15 hrs 12-15 hrs
2 x 150 bp (300 cycles) 16-19 hrs 16-19 hrs
Quality 75-90% Reads > Q30 across all kits
Accuracy 99.6-99.9% across all kits
Number of reads (clusters) 150-165 M
Sequencing Output
(Base calls)
1 x 50 bp (50 cycles)
2 x 50 bp (100 cycles) 15-17 Gb
2 x 100 bp (200 cycles) 30-33 Gb
2 x 150 bp (300 cycles) 45-50 Gb
Run Time 1 x 50 bp (50 cycles)
2 x 50 bp (100 cycles) 8-10 hrs
2 x 100 bp (200 cycles) 12-15 hrs
2 x 150 bp (300 cycles) 16-19 hrs
Quality 70-90% Reads > Q30 across all kits
Accuracy 99.6-99.9% Across F2 and F3 Flowcells
The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
1 Performance metrics may be impacted by sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.
2 The G4 instrument supports two read densities; the F2 and forthcoming F3 flow cells.
3 Kits are available in multiple cycle configurations for application flexibility and can be purchased in quantities of 1 and 4 flow cells.
* Planned for next release

Applications


High sequencing throughput, modular run sizes, and lane-by-lane addressability enables users to process samples across a variety of applications. 

Run Time Samples / Lane Samples / FC Samples / Run
RNA Gene Expression
(2x50 bp, 10M reads)
8-10 hrs 3.75 15 60
Single Cell RNA-Seq
(130 cycles, 7,500 cells/sample and 20,000 reads/cell)
8-10 hrs 0.25 1 4
Total RNA-Seq
(2 x 100 bp, 50M reads)
12-15 hrs 0.75 3 12
Exome
(2 x 100 bp, 35Mb at 100x coverage)
12-15 hrs 1.25 5 20
Target Enrichment
(2 x 150 bp, 800Kb at 4000x coverage)
16-19 hrs 1.5 6 24
Human Whole Genome
(2 x 150 bp, 3Gb at 30x coverage)
16-19 hrs 0.25 1 4
Run Time 8 – 10 Hours
Samples / Lane 3.75
Samples / FC 15
Samples / Run 60
Run Time 8 – 10 Hours
Sample / Lane 0.25
Samples / FC 1
Samples / Run 4
Run Time 12 – 15 Hours
Samples / Lane 0.75
Samples / FC 3
Samples / Run 12
Run Time 12 – 15 Hours
Samples / Lane 1.25
Samples / FC 5
Samples / Run 20
Run Time 16-19 Hours
Samples / Lane 1.5
Samples / FC 6
Samples / Run 24
Run Time16-19 Hours
Samples / Lane0.25
Samples / FC1
Samples / Run4

Sample multiplexing is based on assumptions listed in table and may vary on experimental design and sample type.
*Planned for next release​

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