Demo Data
Download and analyze G4 sequencing data for your application.
Single Cell RNA Sequencing
Overview
We applied the F2 flow cell to perform single cell RNA sequencing on 7,000 fresh frozen healthy donor peripheral blood mononuclear cells (PBMCs) in under 24 hours.
Methods & Analysis
For this experiment, 10x Genomics® Chromium Single Cell 3’ Gene Expression libraries were prepared from 7,000 PBMCs using the 10x Chromium® Next GEM Single Cell 3’ Gene Expression Protocol. Libraries were sequenced with a 28×91 cycle run format. Filtered, paired FASTQs were processed using Cell Ranger count (v6.0.0) and the resulting h5 file was processed using scanpy (v1.8.2).
Results
The scRNA-Seq data generated using the 10x Chromium Single Cell 3’ Gene Expression assay and the G4 Sequencing Platform demonstrates high technical reproducibility and performance comparable to data derived from the Illumina® NextSeq 2000 platform.
RNA Sequencing
Overview
We applied the F2 flow cell to perform RNA sequencing on human RNA samples from Thermo Fisher Scientific Universal Human Reference RNA in under 24 hours.
Methods & Analysis
For this experiment, all human RNA samples were from Thermo Fisher Scientific: Universal Human Reference RNA. Poly(A)-selection steps were carried out per manufacturer guidelines, using 1 μg of input RNA from each sample containing ERCC spike-in Mix 1. Libraries were sequenced with a 2×100 cycle run format. Nf-Core/RNASeq v3.6 was used to perform all downstream bioinformatic processing.
Results
RNA sequencing data generated by the G4 demonstrates performance comparable to the Illumina® NextSeq 2000 platform, in addition to highly correlated gene expression data.
Whole Exome Sequencing
Overview
We applied the F2 flow cell to perform whole exome sequencing of the human reference cell lines HG002-HG006 in under 24 hours.
Methods & Analysis
For this experiment, QuantaBio’s sparQ DNA Frag & Library Prep Kit, 150ng human genomic DNA HG001-HG004 (NIST) was enzymatically fragmented then inactivated. Libraries were individually clustered at 20 pM and sequenced with a 2×155 cycle run format. The data was analyzed via a highly performant DeepVariant model optimized for exome analysis on the G4 Sequencing Platform.
Results
The strong variant detection performance reflects the compatibility of the G4 Sequencing Platform with common exome library preparation kits, but also the suitability of the sequence data for variant detection applications.
Whole Genome Sequencing
Overview
In this application note, we apply the high density F3 flow cell to perform whole genome sequencing of the human reference cell line HG002 in under 24 hours.
Methods & Analysis
For this experiment, 1 ug of gDNA from HG002 was used for PCR-free library preparation via the SparQ Library Preparation Kit with Covaris shearing. The library was quantified via qPCR and 1 μL of sample was PCR amplified to determine library size using an Agilent TapeStation. 40 pM of library was used for onboard amplification of one F3 flow cell followed by sequencing with a 2×150 cycle run format. Google DeepVariant (v1.4.0) and NVIDIA Parabricks were used to analyze the data from the G4 sequencing run.
Results
The G4 Sequencing Platform produced sequencing data on par with current state-of-the-art NGS performance, with single-pass accuracy of ~99.9%, and uniform coverage of the high-confidence regions in the reference genome, all while delivering a rapid turnaround time and flexible throughput.
Innovation with Intention
Featuring novel, high-performance chemistry and advanced engineering, the G4 Sequencing Platform has been optimized to deliver speed, power, unprecedented flexibility, and accuracy.
