Fast, Cost-Effective Single Cell RNA Sequencing

There is a growing desire to answer questions about the genome at the level of the individual cell. Single cell technologies promise to make significant impact on research and clinical studies; however, complimentary high-throughput sequencing for these studies requires large capital expenditure and expensive consumables. The G4™ Sequencing Platform addresses these challenges and enables researchers to generate low-cost, accurate scRNA-seq results with more flexibility and speed than any other sequencer on the market.

Delivering Power and Flexibility for Single Cell Sequencing Labs

The G4 features proprietary 4-color Rapid SBS chemistry and advanced engineering, designed to provide labs with unmatched operational efficiency and industry-leading run times.  The G4 integrates seamlessly into existing NGS workflows and is well suited for single cell sequencing labs.

Key capabilities:

  • Single-day turnaround times
  • Highly accurate data
  • 1 – 4 flow cell, 4 – 16 lane run flexibility
  • Up to 3.2 B reads per run

Highly Accurate Single Cell RNA Sequencing on the G4

  • Highly-accurate single cell data comparable to the NextSeq 2000
  • Rapid and cost-efficient sequencing of single cell RNA sequencing (scRNA-seq) libraries
  • Seamless compatibility of the G4 Sequencing Platform with 10x library preparation workflows and bioinformatics pipelines

Introducing Max Read™ Sequencing Kits

High throughput, low-cost single cell sequencing. 
Up to 3.2 billion reads per run at $1 per million reads.  

G4 Specifications for Single Cell Sequencing

F2 Flow CellF3 Flow CellMax Read Kit2
Reads Delivered / Flow Cell200 M400 M800 M
Reads Delivered / Run800 M1,600 M3,200 M
# Samples / Flow Cell1124
# Samples / Run14816
Price/M Read$3.0$1.8$1.0
Run Time~15 hours~15 hours~24 hours
Quality (Q30)80-90%80-90%80-90%

The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
1Assumes 28×91 read configuration, 10,000 cells/sample and 20,000 reads/cell
2Max Read kits are only compatible with 10x samples and allow for 1 sample per lane. Max Reads cannot be run at the same time as F2 or F3 flow cells

Sample Cost Comparison Tool

Compare flow cell, cost, and run time efficiency against the NextSeq 2000 for your application.

Overview

Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.

Flow Cell Configuration

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • Unused flow cell space
  • Used flow cell space

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F2 Flow Cell
200M Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F3 Flow Cell
400M Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F2 + 1 F3 Flow Cell
600M Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

2 F3 Flow Cells
800M Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F2 + 2 F3 Flow Cells
1B Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

3 F3 Flow Cells
1.2B Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F2 + 3 F3 Flow Cells
1.4B Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

4 F3 Flow Cells
1.6B Reads

  • Flow Cell 1
  • Flow Cell 2
  • Flow Cell 3

Reads
Delivered

NextSeq 2000

G4
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

NextSeq 2000

G4
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

100M reads

NextSeq 2000

G4
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

400M reads

NextSeq 2000

G4
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

1.2B reads

Single Cell

Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.

  • Unused flow cell space
  • Used flow cell space

G4

$200/sample

NextSeq 2000

$833/sample
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

G4 Plug-and-Play Capability

We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.

Talk to an expert about Single Cell RNA Sequencing on the G4 Today

Speak to a member of the Singular team to learn more about the available kits and capabilities for single cell sequencing on the G4. 

Download Single Cell RNA-Seq Application Note

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Download Parse Biosciences Application Note

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Download Single Cell Poster

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Download Takara Bio Application Note

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Talk to an Expert

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Download Demo Data

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Download Application Note

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Max Reads Application Note