Fast, Cost-Effective Single Cell RNA Sequencing
There is a growing desire to answer questions about the genome at the level of the individual cell. Single cell technologies promise to make a significant impact on research and clinical studies; however, complimentary high-throughput sequencing for these studies requires large capital expenditure and expensive consumables. The G4 Sequencing Platform addresses these challenges and enables researchers to generate low-cost, accurate scRNA-seq results with more flexibility and speed than any other sequencer on the market.
Delivering Power and Flexibility for Single Cell Sequencing Labs
G4 features proprietary 4-color Rapid SBS chemistry and advanced engineering, designed to provide labs with unmatched operational efficiency and industry-leading run times. G4 integrates seamlessly into existing NGS workflows and is well suited for single cell sequencing labs.
Key capabilities:
- Single-day turnaround times
- Highly accurate data
- 1–4 flow cell, 4–16 lane run flexibility
- Up to 3.2B paired reads per run
Highly Accurate Single Cell RNA Sequencing on the G4
- Highly-accurate single cell data comparable to the NextSeq 2000
- Rapid and cost-efficient sequencing of single cell RNA sequencing (scRNA-seq) libraries
- Seamless compatibility of the G4 Sequencing Platform with 10x Genomics library preparation workflows and bioinformatics pipelines
Introducing Max Read Sequencing Kits
High throughput, low-cost single cell sequencing.
Up to 3.2 billion paired reads per run at $1 per million reads.
G4 Specifications for Single Cell RNA Sequencing
| F2 Flow Cell | F3 Flow Cell | Max Read2 | ||
|---|---|---|---|---|
| Paired Reads Delivered / Flow Cell | 200M | 400M | 800M | |
| Paired Reads Delivered / Run | 800M | 1.6B | 3.2B | |
| # Samples / Flow Cell1 | 1 | 2 | 4 | |
| # Samples / Run1 | 4 | 8 | 16 | |
| Price / M Paired Read | $3.0 | $1.8 | $1.0 | |
| Run Time | ~15 hours | ~15 hours | ~30 hours | |
| Quality | >85% bases ≥ Q30 | |||
G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
1Assumes 28×91 read configuration, 10,000 cells/sample and 20,000 reads/cell.
2Currently only compatible with 10x Genomics Chromium™ 3’ and 5’ Gene Expression assays and Visium™ Spatial Gene Expression. Kits allow for 1 sample per lane and cannot be run at the same time as F2 or F3 flow cells.
Sample Cost Comparison Tool
Compare flow cell, cost, and run time efficiency against the NextSeq 2000 for your application.
Overview
Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.
Flow Cell Configuration
- 1
- 2
- 3
- 4
- 5
- 6
- Unused flow cell space
- Used flow cell space
G4
G4
1 F2 Flow Cell
200M Reads
G4
1 F3 Flow Cell
400M Reads
G4
1 F2 + 1 F3 Flow Cell
600M Reads
G4
2 F3 Flow Cells
800M Reads
G4
3 F3 Flow Cells
1.2B Reads
G4
4 F3 Flow Cells
1.6B Reads
- P1
- P2
- P3
Reads
Delivered
NextSeq 2000
NextSeq 2000
100M reads
NextSeq 2000
400M reads
NextSeq 2000
1.2B reads
Single Cell
Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000. Click on the flow cell configurations for more information.
Unused flow cell space
Used flow cell space
G4
NextSeq 2000
Overview
Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.
Flow Cell Configuration
- 1
- 2
- 3
- 4
- 5
- 6
- Unused flow cell space
- Used flow cell space
G4
G4
1 F2 Flow Cell
200M Reads
G4
1 F3 Flow Cell
400M Reads
G4
1 F2 + 1 F3 Flow Cell
600M Reads
G4
2 F3 Flow Cells
800M Reads
G4
3 F3 Flow Cells
1.2B Reads
G4
4 F3 Flow Cells
1.6B Reads
- P1
- P2
- P3
Reads
Delivered
NextSeq 2000
NextSeq 2000
100M reads
NextSeq 2000
400M reads
NextSeq 2000
1.2B reads
Single Cell
Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.
- Unused flow cell space
- Used flow cell space
G4
NextSeq 2000
G4 Plug-and-Play Capability
We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.
Talk to an expert about Single Cell RNA Sequencing on the G4 today
Speak to a member of the Singular team to learn more about the available kits and capabilities for single cell sequencing on the G4.
