Preparation FAQsHow to quantify and QC Singular libraries? How much library should be loaded for G4 runs? What are the sequences of Singular adapters? What is the structure of Singular adapters? Does the G4 Sequencing Platform support indexed libraries?
Sequencing FAQsWhat is the expected shelf-life of reagent cartridges? What is the format for the G4 Sample Sheet? What is the Q30 performance for the G4 Sequencing Platform? Does the G4 Sequencing Platform support paired reads (paired-end) sequencing? What are the minimum and maximum read lengths for a sequencing run? How much library should I load per lane onto the G4 Sequencing Platform? What is the ideal sample loading concentration for the G4 Sequencing Platform? What is your recommendation for reagent storage conditions? Is the usage of read length flexible within a run? For example, when using a 100 cycle kit, can I sequence 26 bases on R1 and 74 bases on R2? Can I load different samples with the same index on a single flow cell if the samples are split across different lanes?
Analysis FAQsWhere can I find my raw or demultiplexed/filtered FASTQ files? What are the outputs generated by G4? Where does the demultiplexing take place? How do I re-demultiplex my run? What data format does the G4 Sequencing Platform output?
