G4 Reagents

G4 Sequencing Kits contain all of the necessary reagents for a G4 sequencing run. Kits are available in multiple configurations depending on read length and size requirements for maximum system flexibility and cost efficiency.

Kits & Key Applications

The G4 is a highly versatile system, enabling efficient batching and processing of a wide variety of end user applications.

Run TimeSamples / FCSamples / Run
RNA Gene Expression (2 x 50 bp, 10 M reads)8–10 hrs1560
Single Cell RNA-Seq   (130 cycles, 7,500 cells/sample and 20,000 reads/cell)12-15 hrs14
Total RNA-Seq   (2 x 100 bp, 50 M reads)12–15 hrs312
Exome   (2 x 100 bp, 35 Mb at 100x coverage)12–15 hrs520
Target Enrichment (2 x 150 bp, 800 Kb at 4000x coverage)16–19 hrs624
Run TimeSamples / FCSamples / Run
RNA Gene Expression (2 x 50 bp, 10 M reads)6–8 hrs30120
Single Cell RNA-Seq   (130 cycles, 7,500 cells/sample and 20,000 reads/cell)12-15 hrs28
Total RNA-Seq   (2 x 100 bp, 50 M reads)12–15 hrs624
Exome   (2 x 100 bp, 35 Mb at 100x coverage)12–15 hrs1144
Target Enrichment (2 x 150 bp, 800 Kb at 4000x coverage)16–19 hrs1248
Human Whole Genome (2 x 150 bp, 3 Gb at 30x coverage)16–19 hrs14

Sample multiplexing is based on assumptions listed in table and may vary on experimental design and sample type.
*Planned for next release.

F2 Kit

The F2 Sequencing kits contain all the reagents needed to run the G4, including rapid SBS reagents in 100, 200 and 300 cycle configurations, wash buffer, sample cartridge, and a 150 million read output flowcell. Kits can be purchased in sets of 1 or 4.

150 - 165 M reads (clusters) / FC
ConfigurationOutputApproximate Read TimeProduct Number
1-Pack4-Pack
100 cycles15 - 17 Gb8 - 10 hrs700,101700,104
200 cycles30 - 33 Gb12 - 15 hrs700,102700,105
300 cycles15 - 17 Gb8 - 10 hrs700,103700,106
Quality75 - 90% Reads > Q30 across all kits
Accuracy99.6-99.9% across all kits

*The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.

*Performance metrics may be impacted by sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.

F3 Kit

The F3 Sequencing kits contain all the reagents needed to run the G4, including rapid SBS reagents in 50, 100, 200 and 300 cycle configurations, wash buffer, sample cartridge, and a 300 million read output flowcell. Kits can be purchased in sets of 1 or 4.

300 - 330 M reads (clusters) / FC
ConfigurationOutputApproximate Read TimeProduct Number
1-Pack4-Pack
50 cycles15 - 17 Gb6 - 8 hrsContact UsContact Us
100 cycles30 - 33 Gb8 - 10 hrsContact UsContact Us
200 cycles45 - 50 Gb12 - 15 hrsContact UsContact Us
300 cycles90 - 100 Gb16 - 19 hrsContact UsContact Us
Quality75 - 90% Reads > Q30 across all kits
Accuracy99.6-99.9% across all kits

*The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.

*Performance metrics may be impacted by sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.

Max Read™ Kit

Delivering up to 4 billion reads per run, the Max Read Kits are purpose built to deliver high-throughput, low cost short reads for specific applications.

Indices & Adapters Kit

S1 and S2 sequencing primers are applied during NGS library preparation. Indicies can also be applied during library preparation if within-lane multiplexing is required.

ProductPart Number
Dual Index PCR Primers (1-96)700,110
Dual Index PCR Primers Set A (1-24)700,111
Dual Index PCR Primers Set B (25-48)700,112
SG Non-Indexed Library Prep Primers (24 Reactions)700,115
SG Universal Library Prep Adapters (96 Reactions)700,117
Indexed PCR-Free Library Prep Adapters (24 UDIs x 1 Reaction Each)700,116
Non-Indexed PCR-Free Library Prep Adapters (24 Reactions)700,119

Sequencing Controls

Preprepared PhiX control libraries are recommended for use with the G4 for troubleshooting. These controls can be purchased in sets of 1 or 4.

G4 Pricing & Availability

For additional details around G4 pricing and availability, please provide your information and a Singular Genomics representative will be in contact as soon as possible.