G4 Reagents

G4 Sequencing Kits contain all of the necessary reagents for a G4 sequencing run. Kits are available in multiple configurations depending on read length and size requirements for maximum system flexibility and cost efficiency.

Kits & Key Applications

The G4 is a highly versatile system, enabling efficient batching and processing of a wide variety of end user applications.

F2 Flow Cell
Samples / FC
F3 Flow Cell
Samples / FC
Max Read
Samples / FC
Samples / Run
RNA Gene Expression
(2 x 50 bp, 10M reads)
2040160
RNA-Seq
(2 x 100 bp, 50M reads)
4832
Single Cell RNA-Seq
(130 cycles, 7,500 cells/sample and 20,000 reads/cell)
12416
Exome
(2 x 100 bp, 34Mb at 100x coverage)
61352
Target Enrichment
(2 x 150 bp, 800Kb at 4000x coverage)
81664
Shotgun Metagenomics
(2 x 150 bp, 10M Reads)
2040160
Human Whole Genome2
(2 x 150 bp, 3Gb at 30x coverage)
14
  • Sequencing multiplexing capabilities are approximations based on assumptions and configurations listed in table and expected, but not guaranteed, throughput on G4 flow cells. Results may vary based on experimental design and sample type. Please refer to kit specifications for more detail.

F2 Kit

The F2 Sequencing kits contain all the reagents needed to run the G4, including rapid SBS reagents in 100, 200 and 300 cycle configurations, wash buffer, sample cartridge, and a 150 million read output flow cell. Kits can be purchased in sets of 1 or 4.

Reagent Configurations1Run Time2Reads / Flow Cell3Reads / Run3Quality4
100 Cycle ~11 hrsUp to 250 MUp to 1,000 M80-90% ≥ Q30
200 Cycle~15 hrs
300 Cycle~19 hrs

1 Reagents include 50 additional cycles above what is represented to account for adapters and indices.

2 Run time includes clustering, sequencing and instrument wash for non-indexed reads

3 Paired reads passing filter for F2 and F3 are dependent on application and read length.

4 Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.

F3 Kit

The F3 Sequencing kits contain all the reagents needed to run the G4 Platform, including rapid SBS reagents in 50, 100, 200 and 300 cycle configurations, wash buffer, sample cartridge, and a 300 million read output flowcell. Kits can be purchased in sets of 1 or 4.

Reagent Configurations1Run Time2Reads / Flow Cell3Reads / Run3Quality4
50 Cycle 9-11 hrsUp to 450MUp to 1,800M80-90% ≥ Q30
100 Cycle11-14 hrs
200 Cycle15-19 hrs
300 Cycle19-24 hrs

1 Reagents include 50 additional cycles above what is represented to account for adapters and indices.

2 Run time includes clustering, sequencing and instrument wash for non-indexed reads

3 Paired reads passing filter for F2 and F3 are dependent on application and read length.

4 Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.

Max Read™ Kit

Delivering up to 3.2 billion reads per run, the Max Read Kits are purpose built to deliver high-throughput, low cost short reads for specific applications.

Product1Read / Flow CellQualitySamples per FC2Samples per Run2Run Time
Max Read Single Cell and Spatial Gene Expression800M80-90% ≥ Q30416~24 hrs
Max Read Spatial Gene Expression for FFPE

Max Read Kits contain all components needed to prepare and sequence compatible 10X libraries on the G4, including materials for sample preparation and loading, flow cell, reagent cartridge, and Max Read™ PhiX Control

1Max Read™ products are currently compatible with running 1 sample per lane for 10X Genomics Chromium 3′ and 5′ Single Cell Gene Expression and Visium Spatial Gene Expression Assays

2Assumes 10,000 cell/sample and 20,000 reads/cell for 1 Sample per lane

Library Compatibility Kit

S1 and S2 sequencing primers are applied during NGS library preparation. Indicies can also be applied during library preparation if within-lane multiplexing is required.

ProductPart Number
SG Library Compatibility Kit (24 Reactions)700,141
SG Library Compatibility Kit Sequencing Primers (24 Reactions)700,142

Indices & Adapters

S1 and S2 sequencing primers are applied during NGS library preparation. Indicies can also be applied during library preparation if within-lane multiplexing is required.

Sequencing Controls

Preprepared PhiX control libraries are recommended for use with the G4 for troubleshooting. These controls can be purchased in sets of 1 or 4.

G4 Pricing & Availability

For additional details around G4 pricing and availability, please provide your information and a Singular Genomics representative will be in contact as soon as possible.

Request A Quote

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Max Reads Application Note