G4 Reagents
G4 Sequencing Kits contain all of the necessary reagents for a G4 sequencing run. Kits are available in multiple configurations depending on read length and size requirements for maximum system flexibility and cost efficiency.
Kits & Key Applications
The G4 is a highly versatile system, enabling efficient batching and processing of a wide variety of end user applications.
| F2 Flow Cell Samples / FC |
F3 Flow Cell Samples / FC |
Max Read Samples / FC |
Samples / Run | |
|---|---|---|---|---|
| RNA Gene Expression (2 x 50 bp, 10M reads) |
20 | 40 | — | 160 |
| RNA-Seq (2 x 100 bp, 50M reads) |
4 | 8 | — | 32 |
| Single Cell RNA-Seq (130 cycles, 7,500 cells/sample and 20,000 reads/cell) |
1 | 2 | 4 | 16 |
| Exome (2 x 100 bp, 34Mb at 100x coverage) |
6 | 13 | — | 52 |
| Target Enrichment
(2 x 150 bp, 800Kb at 4000x coverage) |
8 | 16 | — | 64 |
| Shotgun Metagenomics
(2 x 150 bp, 10M Reads) |
20 | 40 | — | 160 |
| Human Whole Genome2
(2 x 150 bp, 3Gb at 30x coverage) |
— | 1 | — | 4 |
- Sequencing multiplexing capabilities are approximations based on assumptions and configurations listed in table and expected, but not guaranteed, throughput on G4 flow cells. Results may vary based on experimental design and sample type. Please refer to kit specifications for more detail.
F2 Kit
The F2 Sequencing kits contain all the reagents needed to run the G4, including rapid SBS reagents in 100, 200 and 300 cycle configurations, wash buffer, sample cartridge, and a 150 million read output flow cell. Kits can be purchased in sets of 1 or 4.
| Reagent Configurations1 | Run Time2 | Reads / Flow Cell3 | Reads / Run3 | Quality4 |
|---|---|---|---|---|
| 100 Cycle | ~11 hrs | Up to 250 M | Up to 1,000 M | 80-90% ≥ Q30 |
| 200 Cycle | ~15 hrs | |||
| 300 Cycle | ~19 hrs |
1 Reagents include 50 additional cycles above what is represented to account for adapters and indices.
2 Run time includes clustering, sequencing and instrument wash for non-indexed reads
3 Paired reads passing filter for F2 and F3 are dependent on application and read length.
4 Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.
F3 Kit
The F3 Sequencing kits contain all the reagents needed to run the G4 Platform, including rapid SBS reagents in 50, 100, 200 and 300 cycle configurations, wash buffer, sample cartridge, and a 300 million read output flowcell. Kits can be purchased in sets of 1 or 4.
| Reagent Configurations1 | Run Time2 | Reads / Flow Cell3 | Reads / Run3 | Quality4 |
|---|---|---|---|---|
| 50 Cycle | 9-11 hrs | Up to 450M | Up to 1,800M | 80-90% ≥ Q30 |
| 100 Cycle | 11-14 hrs | |||
| 200 Cycle | 15-19 hrs | |||
| 300 Cycle | 19-24 hrs |
1 Reagents include 50 additional cycles above what is represented to account for adapters and indices.
2 Run time includes clustering, sequencing and instrument wash for non-indexed reads
3 Paired reads passing filter for F2 and F3 are dependent on application and read length.
4 Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.
Max Read™ Kit
Delivering up to 3.2 billion reads per run, the Max Read Kits are purpose built to deliver high-throughput, low cost short reads for specific applications.
| Product1 | Read / Flow Cell | Quality | Samples per FC2 | Samples per Run2 | Run Time |
|---|---|---|---|---|---|
| Max Read Single Cell and Spatial Gene Expression | 800M | 80-90% ≥ Q30 | 4 | 16 | ~24 hrs |
| Max Read Spatial Gene Expression for FFPE |
Max Read Kits contain all components needed to prepare and sequence compatible 10X libraries on the G4, including materials for sample preparation and loading, flow cell, reagent cartridge, and Max Read™ PhiX Control
1Max Read™ products are currently compatible with running 1 sample per lane for 10X Genomics Chromium 3′ and 5′ Single Cell Gene Expression and Visium Spatial Gene Expression Assays
2Assumes 10,000 cell/sample and 20,000 reads/cell for 1 Sample per lane
Indices & Adapters Kit
S1 and S2 sequencing primers are applied during NGS library preparation. Indicies can also be applied during library preparation if within-lane multiplexing is required.
| Product | Part Number |
|---|---|
| Dual Index PCR Primers (1-96) | 700,110 |
| Dual Index PCR Primers Set A (1-24) | 700,111 |
| Dual Index PCR Primers Set B (25-48) | 700,112 |
| SG Non-Indexed Library Prep Primers (24 Reactions) | 700,115 |
| SG Universal Library Prep Primers (96 Reactions) | 700,117 |
| Indexed PCR-Free Library Prep Adapters (24 UDIs x 1 Reactions Each) | 700,116 |
| Non-Indexed PCR-Free Library Prep Adapters (24 Reactions) | 700,119 |
Sequencing Controls
Preprepared PhiX control libraries are recommended for use with the G4 for troubleshooting. These controls can be purchased in sets of 1 or 4.
G4 Pricing & Availability
For additional details around G4 pricing and availability, please provide your information and a Singular Genomics representative will be in contact as soon as possible.