G4 Reagents
G4 Sequencing Kits contain all of the necessary reagents for a G4 sequencing run. Kits are available in multiple configurations depending on read length and size requirements for maximum system flexibility and cost efficiency.
Kits & Key Applications
G4 is a highly versatile system, enabling efficient batching and processing of a wide variety of end user applications.
| F2 Flow Cell Samples / Flow Cell |
F3 Flow Cell Samples / Flow Cell |
Max Read Samples / Flow Cell |
Samples / Run | |
|---|---|---|---|---|
| RNA Gene Expression (2x50 bp, 10M paired reads) |
20 | 40 | — | 160 |
| RNA-Seq (2x100 bp, 50M paired reads) |
4 | 8 | — | 32 |
| Single Cell RNA-Seq (130 cycles, 7,500 cells/sample and 20,000 paired reads/cell) |
1 | 2 | 4 | 16 |
| Exome (2x150 bp, 34 Mb at 150x coverage) |
17 | 35 | — | 141 |
| Target Enrichment
(2x150 bp, 800 Kb at 4,000x coverage) |
18 | 37 | — | 150 |
| Shotgun Metagenomics (2x150 bp, 10M paired reads) |
20 | 40 | — | 160 |
| Human Whole Genome (2x150 bp, 3 Gb at 30x coverage) |
— | 1 | — | 4 |
- Sequencing multiplexing capabilities are approximations based on assumptions and configurations listed in table and expected, but not guaranteed, throughput on G4 flow cells. Results may vary based on experimental design and sample type. Please refer to kit specifications for more detail.
F2 Kit
The F2 sequencing kits contain all the reagents needed to run the G4 Platform, including rapid SBS reagents in 100, 200 and 300 cycle configurations, wash buffer, sample cartridge, and a 200 million read output flow cell. Kits can be purchased in sets of 1 or 4.
| Reagent Configuration1 | Run Time2 | Paired Reads / Flow Cell3 | Paired Reads / Run3 | Quality4 |
|---|---|---|---|---|
| 100 cycles | ~11 hours | 200M | 800M | >85% bases ≥ Q30 |
| 200 cycles | ~15 hours | |||
| 300 cycles | ~19 hours |
1Reagents include 50 additional cycles above what is represented to account for index sequencing needs.
2Run time is measured from run start through clustering, sequencing, and instrument wash for non-indexed reads.
3Paired reads passing filter for F2 and F3 are dependent on application and read length.
4Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.
F3 Kit
The F3 sequencing kits contain all the reagents needed to run the G4 Platform, including rapid SBS reagents in 50, 100, 200 and 300 cycle configurations, wash buffer, sample cartridge, and a 400 million read output flow cell. Kits can be purchased in sets of 1 or 4.
| Reagent Configuration1 | Run Time2 | Paired Reads / Flow Cell3 | Paired Reads / Run3 | Quality4 |
|---|---|---|---|---|
| 50 cycles | ~11 hours | 400M | 1,600M | >85% bases ≥ Q30 |
| 100 cycles | ~14 hours | |||
| 200 cycles | ~19 hours | |||
| 300 cycles | ~24 hours |
1Reagents include 50 additional cycles above what is represented to account for index sequencing needs.
2Run time is measured from run start through clustering, sequencing, and instrument wash for non-indexed reads.
3Paired reads passing filter for F2 and F3 are dependent on application and read length.
4Performance metrics may be impacted by application, sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.
Max Read™ Kit
Delivering up to 3.2 billion reads per run, the Max Read kits are purpose built to deliver high-throughput, low cost short reads for specific applications.
| Product1 | Run Time2 | Paired Reads per Flow Cell | $ per M Paired Read | $ per Sample | Quality |
|---|---|---|---|---|---|
| Max Read™ Single Cell and Spatial Gene Expression | ~30 hours | 800M | $1.00 | $200 | >85% bases ≥ Q30 |
| Max Read™ Spatial Gene Expression for FFPE | ~24 hours | 1.2B | $0.67 |
Max Read Kits contain all components needed to prepare and sequence compatible 10x Genomics libraries on G4, including materials for sample preparation and loading, flow cell, reagent cartridge, and Max Read PhiX Control.
1Currently compatible with 10x Genomics Chromium™ 3′ and 5′ Gene Expression assays and Visium™ Spatial Gene Expression. Kits allow for 1 sample per lane.
2Assumes 10,000 cell/sample and 20,000 paired reads/cell for 1 sample per lane.
Library Compatibility Kit
S1 and S2 sequencing primers are applied during NGS library preparation. Indices can also be applied during library preparation if within-lane multiplexing is required.
| Product | Part Number |
|---|---|
| SG Library Compatibility Kit (24 Rxns) | 700,141 |
| SG Library Compatibility Kit Sequencing Primers | 700,142 |
Indices & Adapters
S1 and S2 sequencing primers are applied during NGS library preparation. Indices can also be applied during library preparation if within-lane multiplexing is required.
Sequencing Controls
| Product | Part Number |
|---|---|
| SG Universal Library Prep Adapter + UDI Primers [1-96] | 700,110 |
| SG Universal Library Prep Adapter + UDI Primers Set A [1-24] | 700,111 |
| SG Indexed PCR-Free Library Prep Adapter (24 Rxns) | 700,116 |
| SG Non-Indexed Library Prep Primers (24 Rxns) | 700,119 |
| SG UDI Primers [1-96] | 700,134 |
| SG UDI Primers Set A [1-24] | 700,135 |
Preprepared PhiX control libraries are recommended for use with the G4 for troubleshooting. These controls can be purchased in sets of 1 or 4.
G4 Pricing & Availability
For additional details around G4 pricing and availability, please provide your information and a Singular Genomics representative will be in contact as soon as possible.
