Powerful Whole Exome Sequencing for Variant Discovery
Whole exome sequencing (WES) enables the discovery and assessment of genetic variations linked to rare or complex diseases and is a key tool for the diagnosis of genetic disease, population genome studies, and tumor-normal sequencing protocols used in precision oncology. With high accuracy and unparalleled flexibility, the G4 Platform delivers reliable WES results with industry-leading speed.


Unparalleled Speed, Power and Operational Flexibility
The G4 Sequencing Platform offers unmatched flexibility, while delivering faster results with high accuracy. The platform is a plug-and-play solution, seamlessly integrating into existing sequencing workflows and laboratory ecosystems.
Key capabilities:
- Rapid SBS enables cost-efficient overnight delivery of 8–64 exome samples in 16-19 hours.*
- The G4 delivers highly accurate exome data comparable to leading platforms with SNP and INDEL F1scores at 99% and 95% respectively.
*Assuming 34 MB, ~100x coverage
G4 Specifications for Whole Exome Sequencing
F2 Flow Cell | F3 Flow Cell | |
---|---|---|
Reads Delivered / Flow Cell | 200 M | 400 M |
Reads Delivered / Run | 800 M | 1,600 M |
# Samples / Flow Cell1 | 6 | 13 |
# Samples / Run1 | 24 | 52 |
Price/M Read | $3.5 | $2.2 |
Run Time | 15-19 hours | 15-19 hours |
Quality (Q30) | 80-90% | 80-90% |
The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
1Assumes 2×100 read configuration
Performance Characterization of the G4 for Whole Exome Sequencing
The whole exome sequencing application note highlights the capability for the G4 to deliver accurate results comparable with industry-leading platforms with more speed and added flexibility.

Sample Cost Comparison Tool
Compare flow cell, cost, and run time efficiency against the NextSeq 2000 for your application.
Overview
Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.
Flow Cell Configuration
- 1
- 2
- 3
- 4
- 5
- 6
- Unused flow cell space
- Used flow cell space
G4

G4

1 F2 Flow Cell
200M Reads
G4

1 F3 Flow Cell
400M Reads
G4

1 F2 + 1 F3 Flow Cell
600M Reads
G4

2 F3 Flow Cells
800M Reads
G4

3 F3 Flow Cells
1.2B Reads
G4

4 F3 Flow Cells
1.6B Reads
- P1
- P2
- P3
Reads
Delivered
NextSeq 2000

NextSeq 2000

100M reads
NextSeq 2000

400M reads
NextSeq 2000

1.2B reads
Exomes
Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000. Click on the flow cell configurations for more information.
Unused flow cell space
Used flow cell space
G4
NextSeq 2000
Overview
Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.
Flow Cell Configuration
- 1
- 2
- 3
- 4
- 5
- 6
- Unused flow cell space
- Used flow cell space
G4

G4

1 F2 Flow Cell
200M Reads
G4

1 F3 Flow Cell
400M Reads
G4

1 F2 + 1 F3 Flow Cell
600M Reads
G4

2 F3 Flow Cells
800M Reads
G4

3 F3 Flow Cells
1.2B Reads
G4

4 F3 Flow Cells
1.6B Reads
- P1
- P2
- P3
Reads
Delivered
NextSeq 2000

NextSeq 2000

100M reads
NextSeq 2000

400M reads
NextSeq 2000

1.2B reads
Exomes
Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000. Click on the flow cell configurations for more information.
- Unused flow cell space
- Used flow cell space
G4
NextSeq 2000
G4 Plug-and-Play Capability
We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.
Talk to an expert about Whole Exome Sequencing on the G4 Today
Speak to a member of the Singular team to learn more about the available kits and capabilities for Whole Exome sequencing on the G4.