Rapid Metagenomic Sequencing with the G4
Metagenomic next-generation sequencing (mNGS), also known as shotgun sequencing, is considered a “hypothesis-free” approach that enables researchers to identify potential pathogens in a heterogeneous mixture of microbes, through a sensitive and unbiased method. Traditional methods of pathogen identification, such as culture-based methods, can be time-and labor-intensive, resulting in greater delays to prevent spread of diseases caused by specific microbes and advance treatments and therapies. The G4 Sequencing Platform is addressing these challenges by enabling researchers to generate high-quality mNGS results daily with more operational efficiency and lower cost.
Unmatched Speed and Operational Flexibility, Plus Powerful Resolution
The G4 Sequencing Platform delivers high-quality mNGS data for high resolution of complex microbial communities and de novo genome assembly. Unique platform flexibility enables cost-efficient sequencing with unmatched turnaround times.
- Rapid SBS delivers highly accurate reads with single-day turnaround
- Unbiased and reproducible quantification of low and high GC microorganisms
- Plug-and-play compatibility with standard microbial bioinformatics tools
G4 Specifications for Metagenomic Sequencing
| F2 Flow Cell | F3 Flow Cell | |
|---|---|---|
| Reads Delivered / Flow Cell | 200 M | 400 M |
| Reads Delivered / Run | 800 M | 1,600 M |
| # Samples / Flow Cell1 | 20 | 40 |
| # Samples / Run1 | 80 | 160 |
| Price/M Read | $4 | $2.5 |
| Run Time | < 24 hours | < 24 hours |
| Quality (Q30) | 80-90% | 80-90% |
The G4 can process up to 4 flow cells in parallel.
1Assumes 2×150 read configuration
Performance Evaluation of the G4 for Metagenomic Sequencing
The metagenomic sequencing poster highlights the capability for the G4 to deliver accurate and reproducible species quantitation comparable with the NextSeq 2000, with more speed and added flexibility.
Sample Cost Comparison Tool
Compare flow cell, cost, and run time efficiency against the NextSeq 2000 for shotgun metagenomics.
Overview
Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.
Flow Cell Configuration
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- Unused flow cell space
- Used flow cell space
G4
G4
1 F2 Flow Cell
200M Reads
G4
1 F3 Flow Cell
400M Reads
G4
1 F2 + 1 F3 Flow Cell
600M Reads
G4
2 F3 Flow Cells
800M Reads
G4
1 F2 + 2 F3 Flow Cells
1B Reads
G4
3 F3 Flow Cells
1.2B Reads
G4
1 F2 + 3 F3 Flow Cells
1.4B Reads
G4
4 F3 Flow Cells
1.6B Reads
- Flow Cell 1
- Flow Cell 2
- Flow Cell 3
Reads
Delivered
NextSeq 2000
NextSeq 2000
100M reads
NextSeq 2000
400M reads
NextSeq 2000
1.2B reads
Shotgun Metagenomics
Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000. Click on the flow cell configurations for more information.
- Unused flow cell space
- Used flow cell space
G4
NextSeq 2000
Overview
Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.
Flow Cell Configuration
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- Unused flow cell space
- Used flow cell space
G4
G4
1 F2 Flow Cell
200M Reads
G4
1 F3 Flow Cell
400M Reads
G4
1 F2 + 1 F3 Flow Cell
600M Reads
G4
2 F3 Flow Cells
800M Reads
G4
1 F2 + 2 F3 Flow Cells
1B Reads
G4
3 F3 Flow Cells
1.2B Reads
G4
1 F2 + 3 F3 Flow Cells
1.4B Reads
G4
4 F3 Flow Cells
1.6B Reads
- Flow Cell 1
- Flow Cell 2
- Flow Cell 3
Reads
Delivered
NextSeq 2000
NextSeq 2000
100M reads
NextSeq 2000
400M reads
NextSeq 2000
1.2B reads
Shotgun Metagenomics
Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.
- Unused flow cell space
- Used flow cell space
G4
NextSeq 2000
G4 Plug-and-Play Capability
We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.
Talk to an expert about Metagenomic Sequencing on the G4 Today
Speak to a member of the Singular team to learn more about the available kits and capabilities for Metagenomic sequencing on the G4.