Ultimate Flexibility for Your Next RNA-Seq Project

RNA Sequencing (RNA-Seq) is a widely adopted technique for biological research. While innovations over the past decade have decreased the cost-per-sample, researchers are required to multiplex hundreds of samples together to achieve those low costs, resulting in operational inefficiency and long turn-around times. The G4 Sequencing Platform addresses these challenges by enabling researchers to generate fast and accurate RNA-Seq results at a low cost without needing to pool large number of samples.

Innovative G4 Design: Delivering Fast and Flexible RNA Sequencing

Founded on the core principles of accuracy, speed, flexibility, and power – G4 is a versatile and ecosystem compatible benchtop sequencer that is well suited for RNA sequencing.

Key capabilities:

A unique flow cell design and scalable capacity allows labs to process 4–32 samples per run across 1 to 4 flow cells at a time and up to 16 independent lanes.
Produces accurate RNA-Seq data highly correlated with the industry standard benchtop sequencer in less than 24 hours.

*Assumes 25M reads and run times are for 1×50 bp and 2×100 bp run configurations, respectively.

G4 Specifications for RNA Sequencing

G4 can process up to 4 flow cells in parallel. The number of reads and sequencing output shown is per flow cell.
¹Assumes 2×100 bp, 50M read sequencing per sample.

²Run time is dependent on read length.

Performance Characterization of the G4 for RNA-Seq

	F2 Flow Cell	F3 Flow Cell
Paired Reads Delivered / Flow Cell	200M	400M
Paired Reads Delivered / Run	800M	1.6B
# Samples / Flow Cell¹	4	8
# Samples / Run¹	16	32
Price / M Paired Read	$3.5	$2.2
Run Time²	16-18 hours	16-18 hours
Quality	>85% bases ≥ Q30	>85% bases ≥ Q30

The RNA-Seq application note highlights the capability for the G4 to plug into existing NGS library preparation workflows and bioinformatic pipelines to produce highly accurate results.

Sample Cost Comparison Tool

Compare flow cell, cost, and run time efficiency against the NextSeq 2000 for RNA Sequencing.

Overview

Transcriptomes

Overview

Transcriptomes

Overview

Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.

Flow Cell Configuration

Unused flow cell space
Used flow cell space

Reads
Delivered

Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

Transcriptomes

Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000. Click on the flow cell configurations for more information.

Unused flow cell space
Used flow cell space

G4

$110/sample

NextSeq 2000

$342/sample

Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 6 different configurations. See configuration selector for more details.

Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

G4 Plug-and-Play Capability

We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.