Ultimate Flexibility for Your Next RNA-Seq Project
RNA Sequencing (RNA-Seq) is a widely adopted technique for biological research. While innovations over the past decade have decreased the cost-per-sample, researchers are required to multiplex hundreds of samples together to achieve those low costs, resulting in operational inefficiency and long turn-around times. The G4™ Sequencing Platform addresses these challenges by enabling researchers to generate fast and accurate RNA-Seq results at a low cost without needing to pool large number of samples.
Innovative G4 Design: Delivering Fast and Flexible RNA Sequencing
Founded on the core principles of accuracy, speed, flexibility, and power – the G4 is a versatile and ecosystem compatible benchtop sequencer that is well suited for RNA sequencing.
Key capabilities:
- A unique flow cell design and scalable capacity allows labs to process 6 – 48 samples per run across 1 to 4 flow cells at a time and up to 16 independent lanes.
- Produces accurate RNA-Seq data highly correlated with the industry standard benchtop sequencer in less than 24 hours.
*Assumes 25M reads and run times are for 1 x 50 and 2 x 100 run configurations respectively
G4 Specifications for RNA Sequencing
| F2 Flow Cell | F3 Flow Cell | |
|---|---|---|
| Reads Delivered / Flow Cell | 200 M | 400 M |
| Reads Delivered / Run | 800 M | 1,600 M |
| # Samples / Flow Cell1 | 4 | 8 |
| # Samples / Run1 | 16 | 32 |
| Price/M Read | $3.5 | $2.2 |
| Run Time | 16-18 hours | 16-18 hours |
| Quality (Q30) | 80-90% | 80-90% |
The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
1Assumes 2×100 read configuration
Performance Characterization of the G4 for RNA-Seq
The RNA-Seq application note highlights the capability for the G4 to plug into existing NGS library preparation workflows and bioinformatic pipelines to produce highly accurate results.
Sample Cost Comparison Tool
Compare flow cell, cost, and run time efficiency against the NextSeq 2000 for your application.
Overview
Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.
Flow Cell Configuration
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- Unused flow cell space
- Used flow cell space
G4
G4
1 F2 Flow Cell
200M Reads
G4
1 F3 Flow Cell
400M Reads
G4
1 F2 + 1 F3 Flow Cell
600M Reads
G4
2 F3 Flow Cells
800M Reads
G4
1 F2 + 2 F3 Flow Cells
1B Reads
G4
3 F3 Flow Cells
1.2B Reads
G4
1 F2 + 3 F3 Flow Cells
1.4B Reads
G4
4 F3 Flow Cells
1.6B Reads
- Flow Cell 1
- Flow Cell 2
- Flow Cell 3
Reads
Delivered
NextSeq 2000
NextSeq 2000
100M reads
NextSeq 2000
400M reads
NextSeq 2000
1.2B reads
Transcriptomes
Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000. Click on the flow cell configurations for more information.
- Unused flow cell space
- Used flow cell space
G4
NextSeq 2000
Overview
Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.
Flow Cell Configuration
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- Unused flow cell space
- Used flow cell space
G4
G4
1 F2 Flow Cell
200M Reads
G4
1 F3 Flow Cell
400M Reads
G4
1 F2 + 1 F3 Flow Cell
600M Reads
G4
2 F3 Flow Cells
800M Reads
G4
1 F2 + 2 F3 Flow Cells
1B Reads
G4
3 F3 Flow Cells
1.2B Reads
G4
1 F2 + 3 F3 Flow Cells
1.4B Reads
G4
4 F3 Flow Cells
1.6B Reads
- Flow Cell 1
- Flow Cell 2
- Flow Cell 3
Reads
Delivered
NextSeq 2000
NextSeq 2000
100M reads
NextSeq 2000
400M reads
NextSeq 2000
1.2B reads
Transcriptomes
Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.
- Unused flow cell space
- Used flow cell space
G4
NextSeq 2000
G4 Plug-and-Play Capability
We have partnered with industry-leading library preparation providers and bioinformatic pipelines to ensure the G4 Sequencing Platform seamlessly integrates into existing NGS workflows.
Talk to an expert about RNA Sequencing on the G4 Today
Speak to a member of the Singular team to learn more about the available kits and capabilities for RNA sequencing on the G4.