Discover G4

A benchtop sequencing platform delivering accuracy, speed, power, and unprecedented flexibility for a wide range of genomic applications.

G4

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Reimagined Chemistry

At the core of the G4 is a proprietary 4-color Rapid SBS chemistry comprised of novel nucleotides, specifically-engineered enzymes, and molecular biology methods to deliver highly accurate paired-read sequencing.

Flowcell Macro

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4 Lanes per Flow Cell

Each flow cell is intentionally designed with 4 independent lanes, reducing sample pooling and offering flexibility to run multiple experiments in parallel.

Flow Cell Flow Cell Flow Cell Flow Cell

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Up to 4 Flow Cells per Run

The G4 delivers scalable run capacity to empower labs that have variable volume and time-sensitive samples with cost efficient, single-day turnaround times.

Flow Cell
Flow Cell
Flow Cell
Flow Cell

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6-19 Hour Run Times

Novel chemistry combined with breakthrough imaging technology and rapid fluidics reduces run times from days to hours.

G4 G4 Optical Layout

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Unmatched Power in a Benchtop Sequencer

The G4 is engineered for throughput. With up to 3x more data output per hour than the leading benchtop instrument, the G4 will sequence up to 4 human genomes in just 19 hours.

G4

Workflow

The G4 was designed to fit into any existing lab ecosystem. From compatibility with a wide range of sample preparation workflows through seamless integration into bioinformatic pipelines, the G4 is a plug-and-play solution.

Prepare

  • Compatibility with leading library preparation kits and workflows
  • Up to 4 flow cells with 4 individual lanes for maximum run flexibility
  • On and off instrument run creation

Sequence

  • Rapid SBS with four colors
  • Automatic, integrated clustering and instrument wash
  • Real-time remote run and system health monitoring

Analyze

  • FASTQ file output for seamless integration with bioinformatics pipelines
  • Built-in demultiplexing
  • Connectivity for cloud-based storage and remote customer service

G4 Specifications

F2 Kits F3 Kits*
Number of reads (clusters) 150–165 M 300–330 M
Sequencing Output (Base calls) 1 x 50 bp (50 cycles) 15–17 Gb
2 x 50 bp (100 cycles) 15–17 Gb 30–33 Gb
2 x 100 bp (200 cycles) 30–33 Gb 60–66 Gb
2 x 150 bp (300 cycles) 45–50 Gb 90–100 Gb
Run Time 1 x 50 bp (50 cycles) 6-8 hrs
2 x 50 bp (100 cycles) 8-10 hrs 8-10 hrs
2 x 100 bp (200 cycles) 12–15 hrs 12–15 hrs
2 x 150 bp (300 cycles) 16–19 hrs 16–19 hrs
Quality 75–90% Reads > Q30 across all kits
Accuracy 99.6–99.9% across all kits

The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.

  1. Performance metrics may be impacted by sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.
  2. The G4 instrument supports two read densities; the F2 and forthcoming F3 flow cells.
  3. Kits are available in multiple cycle configurations for application flexibility and can be purchased in quantities of 1 and 4 flow cells.
  4. * Planned for next release.

G4 Applications

High sequencing throughput, modular run sizes, and lane-by-lane addressability enables users to process samples across a variety of applications.

Run Time Samples / Lane Samples / FC Samples / Run
RNA Gene Expression (2 x 50 bp, 10 M reads) 8–10 hrs 3.75 15 60
Single Cell RNA-Seq (130 cycles, 7,500 cells/sample and 20,000 reads/cell) 8–10 hrs 0.25 1 4
Total RNA-Seq   (2 x 100 bp, 50 M reads) 12–15 hrs 0.75 3 12
Exome (2 x 100 bp, 35 Mb at 100x coverage) 12–15 hrs 1.25 5 20
Target Enrichment (2 x 150 bp, 800 Kb at 4000x coverage) 16–19 hrs 1.5 6 24
Run Time Samples / Lane Samples / FC Samples / Run
RNA Gene Expression (2 x 50 bp, 10 M reads) 6–8 hrs 7.5 30 120
Single Cell RNA-Seq (130 cycles, 7,500 cells/sample and 20,000 reads/cell) 8–10 hrs 0.5 2 8
Total RNA-Seq (2 x 100 bp, 50 M reads) 12–15 hrs 1.5 6 24
Exome (2 x 100 bp, 35 Mb at 100x coverage) 12–15 hrs 2.75 11 44
Target Enrichment (2 x 150 bp, 800 Kb at 4000x coverage) 16–19 hrs 3 12 48
Human Whole Genome (2 x 150 bp, 3 Gb at 30x coverage) 16–19 hrs 0.25 1 4

Sample multiplexing is based on assumptions listed in table and may vary on experimental design and sample type.
*Planned for next release.

Specialized Applications

HD-Seq

High Definition Sequencing is a unique library preparation and sequencing method for double-stranded DNA, delivering Q50 accuracy and enabling rare variant detection with higher efficiency.

XR-Seq

Extended Range Sequencing is a library preparation and sequence assembly technique facilitating high accuracy targeted reads of up to 3,000 bases.

G4 Pricing & Availability

For additional details around G4 pricing and availability, please provide your information and a Singular Genomics representative will be in contact as soon as possible.