Discover G4
A benchtop sequencing platform delivering accuracy, speed, power, and unprecedented flexibility for a wide range of genomic applications.
Accuracy
Reimagined Chemistry
At the core of the G4 is a proprietary 4-color Rapid SBS chemistry comprised of novel nucleotides, specifically-engineered enzymes, and molecular biology methods to deliver highly accurate paired-read sequencing.
Flexibility
4 Lanes per Flow Cell
Each flow cell is intentionally designed with 4 independent lanes, reducing sample pooling and offering flexibility to run multiple experiments in parallel.
Flexibility
Up to 4 Flow Cells per Run
The G4 delivers scalable run capacity to empower labs that have variable volume and time-sensitive samples with cost efficient, single-day turnaround times.
Speed
6-19 Hour Run Times
Novel chemistry combined with breakthrough imaging technology and rapid fluidics reduces run times from days to hours.
Power
Unmatched Power in a Benchtop Sequencer
The G4 is engineered for throughput. With up to 3x more data output per hour than the leading benchtop instrument, the G4 will sequence up to 4 human genomes in just 19 hours.
Workflow
The G4 was designed to fit into any existing lab ecosystem. From compatibility with a wide range of sample preparation workflows through seamless integration into bioinformatic pipelines, the G4 is a plug-and-play solution.
Prepare
- Compatibility with leading library preparation kits and workflows
- Up to 4 flow cells with 4 individual lanes for maximum run flexibility
- On and off instrument run creation
Sequence
- Rapid SBS with four colors
- Automatic, integrated clustering and instrument wash
- Real-time remote run and system health monitoring
Analyze
- FASTQ file output for seamless integration with bioinformatics pipelines
- Built-in demultiplexing
- Connectivity for cloud-based storage and remote customer service
G4 Specifications
| F2 Kits | F3 Kits* | ||
|---|---|---|---|
| Number of reads (clusters) | 150–165 M | 300–330 M | |
| Sequencing Output (Base calls) | 1 x 50 bp (50 cycles) | 15–17 Gb | |
| 2 x 50 bp (100 cycles) | 15–17 Gb | 30–33 Gb | |
| 2 x 100 bp (200 cycles) | 30–33 Gb | 60–66 Gb | |
| 2 x 150 bp (300 cycles) | 45–50 Gb | 90–100 Gb | |
| Run Time | 1 x 50 bp (50 cycles) | 6-8 hrs | |
| 2 x 50 bp (100 cycles) | 8-10 hrs | 8-10 hrs | |
| 2 x 100 bp (200 cycles) | 12–15 hrs | 12–15 hrs | |
| 2 x 150 bp (300 cycles) | 16–19 hrs | 16–19 hrs | |
| Quality | 75–90% Reads > Q30 across all kits | ||
| Accuracy | 99.6–99.9% across all kits |
The G4 can process up to 4 flow cells in parallel. Number of reads and sequencing output shown is per flow cell.
- Performance metrics may be impacted by sample quality, library preparation, loading concentration, and other sequencing considerations. Metrics as generated on reference bacterial and human genomes.
- The G4 instrument supports two read densities; the F2 and forthcoming F3 flow cells.
- Kits are available in multiple cycle configurations for application flexibility and can be purchased in quantities of 1 and 4 flow cells.
- * Planned for next release.
G4 Applications
High sequencing throughput, modular run sizes, and lane-by-lane addressability enables users to process samples across a variety of applications.
| Run Time | Samples / Lane | Samples / FC | Samples / Run | |
|---|---|---|---|---|
| RNA Gene Expression (2 x 50 bp, 10 M reads) | 8–10 hrs | 3.75 | 15 | 60 |
| Single Cell RNA-Seq (130 cycles, 7,500 cells/sample and 20,000 reads/cell) | 8–10 hrs | 0.25 | 1 | 4 |
| Total RNA-Seq (2 x 100 bp, 50 M reads) | 12–15 hrs | 0.75 | 3 | 12 |
| Exome (2 x 100 bp, 35 Mb at 100x coverage) | 12–15 hrs | 1.25 | 5 | 20 |
| Target Enrichment (2 x 150 bp, 800 Kb at 4000x coverage) | 16–19 hrs | 1.5 | 6 | 24 |
| Run Time | Samples / Lane | Samples / FC | Samples / Run | |
|---|---|---|---|---|
| RNA Gene Expression (2 x 50 bp, 10 M reads) | 6–8 hrs | 7.5 | 30 | 120 |
| Single Cell RNA-Seq (130 cycles, 7,500 cells/sample and 20,000 reads/cell) | 8–10 hrs | 0.5 | 2 | 8 |
| Total RNA-Seq (2 x 100 bp, 50 M reads) | 12–15 hrs | 1.5 | 6 | 24 |
| Exome (2 x 100 bp, 35 Mb at 100x coverage) | 12–15 hrs | 2.75 | 11 | 44 |
| Target Enrichment (2 x 150 bp, 800 Kb at 4000x coverage) | 16–19 hrs | 3 | 12 | 48 |
| Human Whole Genome (2 x 150 bp, 3 Gb at 30x coverage) | 16–19 hrs | 0.25 | 1 | 4 |
Sample multiplexing is based on assumptions listed in table and may vary on experimental design and sample type.
*Planned for next release.
Specialized Applications
HD-Seq
High Definition Sequencing is a unique library preparation and sequencing method for double-stranded DNA, delivering Q50 accuracy and enabling rare variant detection with higher efficiency.
XR-Seq
Extended Range Sequencing is a library preparation and sequence assembly technique facilitating high accuracy targeted reads of up to 3,000 bases.
G4 Pricing & Availability
For additional details around G4 pricing and availability, please provide your information and a Singular Genomics representative will be in contact as soon as possible.
