Applications

The G4 has been designed to deliver fast, flexible sequencing for a range of genomic applications. Explore the data and performance for your applications.

Unmatched Versatility Serving a Broad Range of Applications

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3.2 Billion Reads
up to480 Gb

Icon Speed

<24 Hour Run Times

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1-4 Flow Cells
4-16 Lanes

Explore your Applications

RNA Sequencing

RNA sequencing (RNA-Seq) is used to assess differential gene expression profiles, detect novel transcripts, and characterize new splice variants or cell types. Explore how the reimagined chemistry and advanced engineering of the G4 can improve your RNA sequencing.  

F2 Flow Cell
Samples / FC
F3 Flow Cell
Samples / FC
Samples / Run
4 8 32

(2 x 100 bp, 50M reads)

Single Cell RNA Sequencing

Single cell RNA sequencing (scRNA-Seq) has revolutionized basic and translational research in immunology, developmental biology and cancer by enabling the resolution of distinct cell populations within heterogeneous samples. Explore how the G4 can seamlessly integrate into your scRNA-Seq workflow and accelerate your results without sacrificing accuracy.

F2 Flow Cell
Samples / FC
F3 Flow Cell
Samples / FC
Max Read
Samples / FC
Samples / Run
1 2 4 16

(28×91 configuration, 10,000 cells/sample and 20,000 reads/cell)

Whole Exome Sequencing

Whole exome sequencing (WES) is a next-generation sequencing method used to discover and assess genetic variations linked to rare or complex diseases. Learn how the G4 provides excellent variant-calling accuracy and unmatched speed and versatility to labs performing WES.

F2 Flow Cell
Samples / FC
F3 Flow Cell
Samples / FC
Samples / Run
6 13 52

(2 x 100 bp, 34Mb at 100x coverage)

Whole Genome Sequencing

Whole genome sequencing (WGS) is the process for analyzing the complete genetic makeup of an organism. Learn more about the suitability of the G4 for human whole genome sequencing in the WGS technical report.

F2 Flow Cell
Samples / FC
F3 Flow Cell
Samples / FC
Samples / Run
1 4

(2 x 150 bp, 3Gb at 30x coverage)

Sample Cost Comparison Tool

Compare flow cell, cost, and run time efficiency against the NextSeq 2000 for your application.

Overview

Click on the flow cell configurations for more read details. Select your application and number of samples to compare flow cell, cost, and run time efficiency against the NextSeq 2000.

Flow Cell Configuration

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • Unused flow cell space
  • Used flow cell space

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F2 Flow Cell
200M Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F3 Flow Cell
400M Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F2 + 1 F3 Flow Cell
600M Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

2 F3 Flow Cells
800M Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F2 + 2 F3 Flow Cells
1B Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

3 F3 Flow Cells
1.2B Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

1 F2 + 3 F3 Flow Cells
1.4B Reads

G4

G4
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.

4 F3 Flow Cells
1.6B Reads

  • Flow Cell 1
  • Flow Cell 2
  • Flow Cell 3

Reads
Delivered

NextSeq 2000

G4
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

NextSeq 2000

G4
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

100M reads

NextSeq 2000

G4
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

400M reads

NextSeq 2000

G4
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

1.2B reads

Shotgun Metagenomics

Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.

  • Unused flow cell space
  • Used flow cell space

G4

$100/sample

NextSeq 2000

$454/sample
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

Transcriptomes

Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.

  • Unused flow cell space
  • Used flow cell space

G4

$198/sample

NextSeq 2000

$342/sample
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

Exomes

Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.

  • Unused flow cell space
  • Used flow cell space

G4

$110/sample

NextSeq 2000

$342/sample
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

Small RNA

Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.

  • Unused flow cell space
  • Used flow cell space

G4

$75/sample

NextSeq 2000

$156/sample
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

Single Cell

Select your application and number of samples to compare flow cell, cost, and run time efficiency against the leading benchtop sequencer. Click on the flow cell configurations for more information.

  • Unused flow cell space
  • Used flow cell space

G4

$200/sample

NextSeq 2000

$833/sample
Flow Cell Configuration (Run Sizes)?G4 is capable of running 1-4 flow cells and 8 different configurations. See configuration selector for more details.
Flow Cell Configuration (Run Sizes)?NextSeq 2000 is capable of running 1 flow cell at a time, with three different flow cell options.

Innovation with Intention

Featuring novel, high-performance chemistry and advanced engineering, the G4 Sequencing Platform has been optimized to deliver speed, power, unprecedented flexibility, and accuracy.

1F2 and F3 Flow cell run combinations and resulting 8 run sizes are in development and not currently available to customers; please contact your sales representative for more information

Download RNA-Seq App Note

Please take a moment to provide some information prior to downloading the RNA-Seq App note.

Download Single Cell RNA-Seq Application Note

Please provide your information to download the app note.

Download Whole Exome Sequencing App Note

Please provide your information to download the exome sequencing app note.

Download WGS Tech Report

Explore and download the G4 Sequencer’s coverage, quality, accuracy and variant calling performance for Whole Genome Sequencing.

Max Reads Application Note